LABORATORIO CLÍNICO - Association between microRNA single nucleotide polymorphisms and the risk of hepatocellular carcinoma

Abril 2016

LABORATORIO CLÍNICO 508 Rev Med Chile 2016; 144: 508-515 1Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China. 2Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, China. 3Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China. 4Key Laboratory of Medical Molecular Virology of MOE/ MOH and Collaborative Innovation Center of Genetics and Development, Institutes of Biomedical Sciences and Department of Immunology of Shanghai Medical School, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China. #These authors contributed equally to this work. Fundings: This work was supported by the grant fromthe Ministry of Science and Technology of China (No. 2012CB910104). Recibido el 21 de octubre de 2015, aceptado el 21 de enero de 2016. Corresponding author: Jun Zhang, Ph.D. Department of Digestive Diseases, Huashan Hospital, Fudan University. 12 Middle Wulumuqi Rd. Shanghai 200040 China Tel & Fax: 86-21-52888236 archsteed@gmail.com Association between microRNA single nucleotide polymorphisms and the risk of hepatocellular carcinoma WENSHUAI LI1#, YANYUN MA2#, DEQING ZENG1, JUN ZHANG1*, RUI WANG1,2, JINGYI HU1, DONGQIN YANG1, HEPING HU3, JIUCUN WANG2, JIE LIU1,4 ABSTRACT Background: Hepatocellular carcinoma (HCC) has a high morbidity and mortality. Single nucleotide polymorphisms (SNPs) of microRNA (miRNA) may be associated with the susceptibility to develop certain malignant tumors. Aim: To study the association between SNPs of miRNA and hepatocellular carcinoma in peripheral blood samples. Material and Methods: Three SNPs in miRNA were studied in peripheral blood samples of 498 patients with HCC and 520 controls. Results: A significant association was observed between rs13299349 in miRNA3152 and HCC. AA genotype or A allele were significantly associated with increased risk of HCC. A allele was associated with the size and number of tumor foci. There was also a relationship between rs10061133 in miRNA449b and HCC. The G allele was significantly associated with increased risk of HCC compared with A allele. Conclusions: This study links rs13299349 in miRNA3152 and rs10061133 in miRNA449b with the risk of developing HCC. (Rev Med Chile 2016; 144: 508-515) Key words: Carcinoma, Hepatocellular; MicroRNAs; Polymorphism, Genetic; Polymorphism, Single Nucleotide. Asociación entre polimorfismos de un nucleótido en microRNA circulante y riesgo de carcinoma hepatocelular Antecedentes: El carcinoma hepatocelular (CHC) tiene una alta morbilidad y mortalidad. Polimorfismos de un nucleótido (SNP) presentes en el microRNA (miRNA) circulante pueden asociarse a ciertos tumores. Objetivo: Estudiar la asociación entre la presencia de SNPs en miRNA circulante y la presencia de carcinoma hepatocelular. Material y Métodos: Se determinó la presencia de tres SNP en microRNA de sangre periférica en 498 pacientes con CHC y 520 controles. Resultados: El SNP rs13299349 en el miRNA3152 se asoció con CHC. El genotipo AA o el alelo A se asociaron con un riesgo mayor de presentar un CHC. El alelo A se asoció además con el tamaño y número de focos del tumor. Se observó también una relación entre el SNP rs10061133 en el miRNA449b y HCC. En este caso, el alelo G se relacionó con un mayor riesgo de CHC. Conclusiones: Los SNP rs13299349 en el miRNA3152 y rs10061133 en el miRNA449b se asocian al riesgo de desarrollar CHC.


Abril 2016
To see the actual publication please follow the link above